Basic research
Abstract Regenerative Restoration Therapy is a clinical technique of Human Body Regenerative Restoration Science, which was widely used in treating refractory ulcers wounds. But due to the differences of the wound in terms of pathogenesis and conditions as well as no unified standard clinical procedures, it resulted in difference of clinical results. In order to unify and standardize the clinical procedures of Regenerative Restoration Therapy in treating the refractory ulcers and wounds the clinical standardized procedures were summarized. On the theoretical and scientific basis of Human Body Regenerative Restoration Sciences founded by Profession Xu Rongxiang, referring to "Clinical Handbook of Regenerative Medicine and Therapy", the clinical standard guideline of treating refractory wounds and ulcers with Regenerative Restoration Therapy was summarized. Guided by Human Body Regenerative Restoration Science and other relevant clinical therapeutic technique this clinical guideline highlights the clinical standardized practice. And this clinical guideline of treating chronic ulcers has reference significance to unify and to standardize the treatment of refractory wounds and ulcers.
Regenerative Restoration Therapy is the clinical therapeutical technique in the Human Body Regenerative Restoration Science. Its definition is to make use of cell-cultivation technique and corresponding clinical therapeutical technique to in situ cultivate Potential Regenerative Cells (PRCs) and realize the regenerative restoration of defected, infected and aged tissues and organs. It is the mainstay medical technique of regenerative medicine.
The cell-cultivation technique in Regenerative Restoration Therapy is the core technique of Human Body Regenerative Restoration Science---"Somatic cells are induced into stem cells that in situ regeneratively restore tissues and organs"1. And clinical therapeutical techniques includes eight techniques: activation and modulation of stem cellsin situ cultivation of stem cellsliquefaction and discharging of necrotic tissues without further damageexogenous culture medium of tissue regenerativephysiological control of bacterial growth and toxin releasingphysiologically moisture environment of tissue regenerationmicro-isolation and of tissue regeneration and inner feedingand connection of tissues into organ2.
Various kinds of wounds and ulcers are common and intractable disorders with high incident rate. Many patients have long-term course of disease with difficulties to be cured for years, even dozens of years. It has little effects to treat these kinds of wounds with routine surgical procedures. It is becoming one of severe social problems for the difficulties in the treatment. In the recent ten years, large numbers of clinical reports showed that regenerative therapy has great clinical efficacy in the treatment of various kinds of refractory wounds. In this article, the mechanism and therapeutical key-points of regenerative restoration therapy treating refractory wounds and ulcers will be given in accordance with the theory and method of Human Body Regenerative Restoration Science.
1. The mechanism of regenerative restoration therapy treating refractory wounds and ulcers
1.1. Maintenance of physiologically moisture environment
1.1.1. The physiologically moisture environment is built up for the wounds and ulcers to extremely decrease the water evaporation from the wound, protect the remnant epithelial tissues on the wound and create the conditions for the regenerative repair of wound.
1.1.2. The physiologically moisture environment of wound facilitates the initiation of biochemical reactions (hydrolysis, enzymolysis, rancidity, saponification, lipidation and esterification. With the discharging of necrotic tissues on the wound in the manner of liquefaction from outside to inside, it will be favor for the healing of the wound by the way of debriding high-glucose exudates via local dressing-change.
1.1.3. The physiologically moisture environment is the key part in the process of activating, initiating Potential Regenerative Cells (PRCs) that lie in the deep layer of wound to transform into stem cells that physiologically regenerate and restore wound. It will accelerate the healing of the wound.
1.2. Prevention and control of infection
1.2.1. Timely control the micro-organism ecology, suppress the rate of bacterial growth, make bacterial mutated and decrease the number of bacterial and the production of endotoxin.
1.2.2. Build up moisture environment to protect and isolate wound.
1.2.3. Moisturize and liquefy necrotic tissues in advance.
1.2.4 Separate the parabionic tissues (the layer between necrotic tissues and unaffected tissues) in advance without damage to the healthy tissues.
1.2.5. Timely administer active drainage.
1.3. Nourishing and activating function
MEBO Wound Ointment and MEBO Wound & Ulcer Dressing are the culture medium to the wound regeneration with abundant essential non-saturated lipid acids (physterol, oleic acid, linoleic acid) and Vitamin E. These active ingredients not only provide the nutrients for the cell regeneration on the base of wound, but also persistently activate the tissues and cells regeneration to make them unceasingly differentiate epithelial cells, fibroblasts and vascular endothelial cells to promote the repairing of tissues and cells; meanwhile, it also can be considered as debriding agent. As the active drainage function can be produced after tropical administration, it not only encircles the granules of necrotic tissue and contaminated particles on the wound, but also timely discharges those contaminated particles, foreign bodies and necrotic tissues out of the MEBO ointment layer in the manner of active drainage. Finally, it attains the results to isolate and protect the fresh wounds and prevent the wound from second contamination.
1.4. Fine analgesic effects
1.4.1. Protect nerve endings;
1.4.2. Relax the spasm of arrectores pilorum;
1.4.3. Restore the blood circulation.